AcroPrep™ Advance 96-Well Filter Plates for DNA Purification product photo Primary L

AcroPrep™ Advance 96-Well Filter Plates for DNA Purification

Maximum Yields of High Quality DNA

  • Optimized for maximum binding yield of DNA from a variety of sample types
  • New well geometry results in reduced hold-up volume and higher recovery of DNA
  • New outlet tip geometry provides direct flow of samples into receiver plate without concerns of crosscontamination
  • Enhanced membrane ensures high yields of contaminant-free DNA for downstream applications
  • Manufactured in accordance with SBS guidelines, allowing entire DNA purification process to be performed on automated equipment


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描述

Maximum Yields of High Quality DNA

  • Optimized for maximum binding yield of DNA from a variety of sample types
  • New well geometry results in reduced hold-up volume and higher recovery of DNA
  • New outlet tip geometry provides direct flow of samples into receiver plate without concerns of crosscontamination
  • Enhanced membrane ensures high yields of contaminant-free DNA for downstream applications
  • Manufactured in accordance with SBS guidelines, allowing entire DNA purification process to be performed on automated equipment
Applications
  • Plasmid DNA purification
  • Genomic DNA purification
Specifications

Materials of Construction

  • Filter Media: DNA binding (borosilicate glass without binder) media
  • Plate Housing: Polyproylene
  • Lid: Polystyrene

Dimensions

  • Length: 12.8 cm (5.0 in.)
  • Width: 8.6 cm (3.4 in.)
  • Height With Lid: 1.8 cm (0.7 in.) (350 μL only)
  • Height Without Lid: 350 μL, 1.4 cm (0.6 in.); 1 mL: 3.3 cm (1.3 in.)

Well-Bottom Area

  • 0.25 cm2

Recommended Working Volume

  • 350 μL: < 300 μL
  • 1 mL: < 900 μL

Recommended Operating Vacuum

  • > 25.4 cm Hg (10 in. Hg)

Recommended Centrifugal Force

  • 1,500 x g

Typical Processing Time

  • Vacuum: 2 seconds
  • Centrifuge: < 2 minutes

Typical Hold-Up Volume

  • Optimized for maximum binding Vacuum: 19 μL
Performance

Plasmid DNA Yield Superior to Competitive Plates



pCAT plasmid DNA yield (OD260) using indicated DNA purification plates starting with 1 mL (Pall and Competitor B) or 1.5 mL (Competitor A) overnight culture DH5α. Purification using plate manufacturer’s recommended protocol using 350 μL plates. Error bars indicate standard error (n > 6).

Quality DNA Consistently Purified

 
  Elution Volume (µL) Recovered Volume (µL) OD260/280 Concentration (ng/µL) Total Yield (µg/well)
Pall 70 55-65 1.95 ± 0.01 131 7.7
Competitor A 50 30 1.98 ± 0.01 155 4.6
Competitor B 70 45-60 2.04 ± 0.05 108 5.6
 
OD260/280 of pooled purified pDNA (n > 6). High quality DNA has an OD260/280 value between 1.7-2.0.
 
Type
Filter Plate
Application
Plasmid DNA purification
Segments
Analytical QC
Segment
Analytical QC
Protein Sample Prep & Detection